Archives for July 12, 2013

Cheung Lab, Days 5 and 6


Leaf discs after being transferred to new medium

This week was a hectic one with my Nanotechnology Class, but I was still able to work in the lab a couple of days, Monday and Friday.  It was similar to other processes that we completed earlier, so it was relatively easy on the “learning” side.

On Monday, we dipped more Arabidopsis plants with 3 different bacterial lines.  When I got to the lab, the plants were already cleaned, so it was as easy as spinning down the bacteria, preparing the solutions, and actually dipping the buds.  We then tied up the plants we had dipped the previous week.


Tied Arabidopsis plants

On Friday, we worked on transferring the tobacco leaf discs from their medium to a new medium; one that induced shoots to grow and selected for antibacterial resistance.  The process was simple- consolidating 3 plates from the previous medium to 2 plates of the new medium.  After transferring the plates, we looked at our direct-soil germinated plants, and spread them out evenly in each plant pot.



Sorting soil-germinated plants

Nanotechnology Institute, Day 5

Gel Diffusion lab, red food coloring

Day 5 was a good conclusion to the excellent experience that I’ve had at the UMass STEM Nanotechnology Institute 2013.  We reviewed our crystal and Gelatin Diffusion experiments with the ADI software that I learned about at STEM Digital.  It was powerful to be able to analyze the results with both Microsoft Excel and the ADI Software.

Jonathan Rothstein gave a presentation and talked further about Nanomedicines.  As I posted yesterday about Nanotechnology impacts, nanomedicine will change the way that we treat many conditions and diseases.  Nanomedicine will change the way that we even think about different diseases.

We spent the rest of the day sharing how we will use the material that we gained from this experience in our classrooms this upcoming school year.  I’ve shared my poster below.